Effect of AC-PACs on C. albicans biofilm desorption. Newly formed biofilms (48 h) of C. albicans biofilms were treated (30 and 120 min) with AC-PACs prior to determine biofilm biomass by staining with crystal violet. Assays were done in triplicate and the means ± SD from three independent experiments were calculated. A value of 100% was assigned to the preformed biofilm unexposed to AC-PACs. *, significantly lower than the value for the unexposed control (P < 0.05).