Figure 5

Significantly transient synergistic relationships between ACA and CDDP, suggesting that ACA plays a role in HSC-4 chemo-sensitization. (A) Isobologram IC₅₀ analysis of HSC-4 cells after exposure to both ACA/CDDP simultaneous and sequential combinations over 48 h. C1: 24 h simultaneous treatment (constant ACA); C2: 24 h simultaneous treatment (constant CDDP); C3: 48 h simultaneous treatment (constant ACA); C4: 48 h simultaneous treatment (constant) CDDP; C5: 12 h pre-ACA prior to 24 h of CDDP sequential treatment; C6: 12 h pre-ACA prior to 48 h of CDDP sequential treatment. (B) Summary of IC₅₀ and combination index (CI) values calculated from in vitro MTT cytotoxicity assays on various treatment combinations. Data were presented as mean ± SEM of independent triplicate experiments.