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Table 2 Genotoxicity induced by extracts from D. gnidium leaves

From: Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts

Extracts

Dose (μg/assay)

β-gal (UE)

PA (UE)

IF

Nitrofurantoin

5

42,18 ± 0,092

11,19 ± 0,0021

23,56

NC

0

2,28 ± 0,031

11,27 ± 0,017

1

Petroleum ether extract

50

2,26 ± 0,043

13,95 ± 0,017

0,81

 

250

1,96 ± 0,002

14,35 ± 0

0,68

 

500

2,13 ± 0,012

13,51 ± 0,01

0,79

Chloroform extract

50

1,27 ± 0,004

8,13 ± 0,016

0,62

 

250

1,52 ± 0,002

8,73 ± 0,007

0,69

 

500

3,28 ± 0,015

11,27 ± 0

1,16

Ethyl acetate extract

5

0,83 ± 0,001

5,11 ± 0,002

0,73

 

15

0,80 ± 0

6,05 ± 0,017

0,60

 

30

1,37 ± 0,002

8,15 ± 0,026

0,76

Methanol extract

50

0,71 ± 0,041

5,45 ± 0,003

0,59

 

100

0,88 ± 0,004

5,92 ± 0,009

0,68

 

200

1,31 ± 0,003

6,58 ± 0,017

0,90

TOF extract

5

1,88 ± 0

5,89 ± 0,003

2

 

15

1,81 ± 0,002

7,98 ± 0,024

1,4

 

30

1,35 ± 0,004

6,58 ± 0,024

1,28

  1. β-gal: units of β-galactosidase; AP: units of phosphatase alkaline; IF: induction factor.
  2. Positive control of genotoxicity (nitrofurantoin); NC: negative control (non-treated cells).