Figure 3

Light microscope images of the wound closure in vitro using confluent monolayer of NIH 3T3 fibroblasts. Microphotographs showing one representative experiment of the cell migration into the created wound area in response to the treatment. (A) Wound area immediately after wounding and (B) after 24 h for the untreated control (medium only, set to 0%); (C) confluent area without wounding (set to 100%) as well as treated areas with substances at 1:100 dilution: succussed solvent 0712–1 (E), remedy 0712–2 (F) and unsuccussed solvent 0712–3 (G) after 24 h incubation. DMEM with 5% FCS (D) was used as positive control. Wound closure (indicated in%) was normalized to the untreated control (B) and the confluent area (C).