Figure 8

Apoptosis of breast and prostate cancer cells by SC/D-F9, tamoxifen and paclitaxel. MCF-7 (A-C) and MDA-MB-231 (D-F) cells were treated with DMSO (0.1%), SC/D-F9 (8.5 or 10.0 μg/ml, repectively), and tamoxifen (15 μM) for 24 hr while the PC-3 (G-I) and DU-145 (J-L) cells were treated with DMSO (0.1%), SC/D-F9 (7.4 and 7.2 μg/ml, respectively) and paclitaxel (50 nM) for 48 hr. The cells were stained with annexin V-FITC antibody (green staining) and propidium iodide (red staining) and observed using a fluorescence microscope (20× magnification).