Fig. 4

Toll-like receptor signaling pathway is activated after HLJD treatment. (A) RNA-seq analysis was performed and the volcano plot was demonstrated. (n = 3 mice/group) (B) Interaction net of the significant pathways (Path-Net) of differentially signaling pathways. (C) Immunohistochemistry was used to analyze the expression levels of TLR7 (400×). HLJD = Huang Lian Jie Du Decoction, ICIs = anti-PD-1 + anti-CTLA-4