Fig. 4
Protective effects of G. lucidum-fermented crop extract B against H2O2- or Aβ25-35-induced apoptosis in SH-SY5Y cells. A The representative image of non-treated SH-SY5Y cells stained with Hoechst 33,342 is shown. The experimental setup involved pretreating the cells with G. lucidum extract B at two different concentrations, namely 50 μM or 100 μM, for a duration of 6 h. Subsequently, the cells were exposed to either (B, C, D) H2O2 (150 μM) or (E, F, G) Aβ25-35 (10 μM) for an additional 24 h. Morphological apoptosis was determined by staining with Hoechst 33,342. Arrow heads indicate apoptosis cells, with condensed chromatin, causing an increase in fluorescence intensity. The scale bar represented 50 μm