Fig. 2
The protective effects of G. lucidum-fermented crop extracts against H2O2-induced cytotoxicity in SH-SY5Y cells were evaluated. The cells were pre-treated with various substances: (A) Crop medium extract, (B) G. lucidum-fermented crop extract A, (C) extract B, (D) extract C, and (E) GABA standard (at concentrations ranging from 10 to 500 μg/mL) for 6 h, followed by treatment with H2O2 (150 μM) for an additional 24 h. Cell viability was assessed using the MTT assay, as detailed in the Materials and methods section. The data were expressed as mean ± SD from three independent experiments. Different letters (a-e) displayed within the same figure indicate significant differences (p < 0.05) among the various treatment groups