Fig. 5

MTS Analysis. MTS analysis of cell viability at 48 and 72 hrs upon treatment with chili extracts (HR, HMR, TMS, J, S, HRS, BJ, JR) and capsaicin (CAP), dihydrocapsaicin (DHK) in different concentrations cultivated with HCT116 cells. The extracts of peppers HR, HMR, TMS, J, SP, HRS, BJ, JR. Control group (cells HCT116) was not affected by extracts, Cap1 - Cap6 concentrations of CAP at 10 μM, 25 μM, 50 μM, 100 μM, 150 μM, 200 μM. DHK1 - DHK6 concentrations of DHK at 10 μM, 25 μM, 50 μM, 100 μM, 150 μM, 200 μM. MTS analysis expressed as a fold of control [%] of absorbance generated in cell-mediated MTS assays to control group. Absorbance values are obtained from three independent experiments (n = 3 for each group) and values are expressed in mean ± SE. The groups treated with extracts alone were compared with control: * p < 0.05, **p < 0.01, ***p < 0.001. * vs Ctrl, ▲ vs HR and each extract mutually at both analyzed times (48 hrs, 72 hrs). Statistically insignificant [ns]: CAP1 vs DHK1, HR vs DHK3 at 48 hrs; CAP1 vs DHK1, BJ vs CAP3, HR vs DHK3, HRS vs JR at 72 hrs