Fig. 2

Decatenation assay for topoisomerase II. Topoisomerase II decatenation assay was carried out using kinetoplast DNA (kDNA, 200 ng) in human topoisomerase IIa (hTop IIa, 1.5 U) enzyme, in 1/10 of stock solutions (3 μL from extracts, capsaicin 0.5 mg/mL and 1.0 mg/mL). Extracts: HR – Habanero Red; HMR – Habanero Maya Red; TMS – Trinidad Moruga Scorpion; J – Jalapeno; SP – Serrano pepper; HRS – Habanero Red Savina; BJ – Bhut Jolokia; JR – Jamaica Rosso (final concentration 1/10 of stock solution); CAP 1 – capsaicin (final concentration 0.05 mg/mL); CAP 2 – capsaicin (final concentration 0.1 mg/mL); DHK – dihydrocapsaicin (final concentration 0.05 mg/mL); K1 – kDNA; K2 – kDNA + Topo II (1.5 U) + EtOH