Fig. 6

Western blot analysis of apoptosis related proteins in HaCaTras II4 cells. a Effect of the F2 fraction on the expression level of p21, p53, caspase-3, Bcl-2 and Bcl-2-associated X (BAX) proteins. Expression of β-actin was used as an internal control. Cells were treated with 25 and 50 μg/ml of F2-fraction or with 0.5% DMSO for 48 h. Western blots are representative of three independent experiments. b The densitometer-intensity data of the proteins of each blot is presented as mean ± SEM from three independent experiments. * denotes P < 0.05 and ** denotes P < 0.01 vs. DMSO as measured by one-way ANOVA