From: Isolation of alpha-linolenic acid from Sutherlandia frutescens and its inhibition of Mycobacterium tuberculosis’ shikimate kinase enzyme
Time (min)
Flow (mL/min)
% HOH
% MeOH
% 0.1 % FA HOH
% CAN
0.0
5.5
0
65
35
2.0
15.0
75
12
13
25.0
100
40.0
44.0
50.0