Fig. 7

Huh-7 cells were culture in 6-well plates and transfected with either fluorescein-conjugated siRNA, non-specific miRNA (NS-miRNA) or miRNA-22 for 72 h as described in Methods. a The cells were observed under the fluorescent microscope (i) and the same field was observed under phase-contrast microscope (ii). b Total RNA was isolated, cDNA was synthesized and real time RT-PCR was performed for miRNA-22 and 5S RNA. The relative expression was calculated and presented (n = 3; p < 0.001). c MiRNA-22 over-expressing cells were analyzed for the proliferation assay using MTT (n = 3; p < 0.01). d The total cellular protein was isolated from miRNA-22 over expressing cells and Western blot was performed for c-myc and b-actin protein. The picture is a representative of three experiments. Lane 1, control; lane 2, NS-miRNA; lane 3, miRNA-22