Fig. 1

Effects of LA on adipogenesis in 3 T3-L1 preadipocytes. a Chemical structure of LA. b 3 T3-L1 preadipocytes were treated with various concentrations of LA during differentiation. On day 8, the differentiated cells were stained with Oil-red-O dye and visualized under inverted microscopy at 100× magnification. c The cellular lipid retained Oil-Red-O in isopropanol and adipocyte expression was estimated by measuring O.D. with a microplate reader (Sunrise, TECAN, Mannedorf, Switzerland) at 510 nm. ### 0.001 < P (compared to preadipocte) and ** P < 0.01 (compared to adipocyte). Data are expressed as mean ± SD. d Total protein prepared from LA-treated 3 T3-L1 (preadipocytes or adipocyte) cells were subjected to western blot analysis for AMPK, p-AMPK, PPARγ, C/EBPα, adiponectin, FAS, SREBP-1, HMGCR, and β-actin. e HPLC analysis of LA in EPK. Preadipocyte (P)