Table 1 Mouse primers for real-time quantitative PCR assays
From: Emodin enhances osteogenesis and inhibits adipogenesis
Genes | Forward primer | Reverse primer |
|---|---|---|
β-actin | 5′-CTTAGTTGCGTTACACCCTTTCTTG-3′ | 5′-CTGCTGTCACCTTCACCGTT CC-3′ |
ALP | 5′-CATGAAATACGAGATCCACCGAGAC-3′ | 5′-ATGCGACCACC CTC CACGAAG-3′ |
Runx2 | 5′-CATGGTGGAGATCATCGC-3′ | 5′-ACT CTT GCC TCG TCC ACT C-3′ |
Osterix | 5′-GCA GCTAGAAGGGCGTGGTG-3′ | 5′-GCAGGCAGGTGAACTTCTTC-3′ |
OC | 5′-GAGGGCAGCGAGGTAGTGAAG-3′ | 5′-GATGTGGTCAGCCAACTCGTCA-3′ |
ColI | 5′-TCCGACCTCTCTCCTCTGAA-3′ | 5′-GAGTGG GGTTATGGAGGGAT-3′ |
BMP4 | 5′-TGAGCCTTTCCAGCAAGTTT-3′ | 5′-CTTCCCCGTCTCAGGTATCA-3′ |
PPARγ | 5′-CCAGAAAGCGATTCCTTCAC-3′ | 5′- CACGTTAGTTTCACCTCGGA-3′ |
C/EBPα | 5′-AAGAAGTCGGTGGACAAGAACAG-3′ | 5′-TGCGCACCGCGATGT-3′ |
ap2 | 5′-GGCCAGGAATTTGACGAAGTC-3′ | 5′-AGCGTAACTTGAGATGTTGTAAGACA-3′ |