Figure 3

Effect of COE on MMP-2 and MMP-9 activity in IL-1β and TNF-α-induced FLSs. FLSs were pretreated with the indicated concentrations of COE for 1 h followed by IL-1β (10 ng/ml) and TNF-α (10 ng/ml) stimulation. After 48 h, the supernatants were collected and assayed for the amount and activity of secreted MMP-2 and MMP-9, by ELISA (A and B), western blot (C and D) and gelatin zymography (E) respectively. The histogram shows the ELISA and western blot results from three independent experiments. ^* P < 0.05, ^** P < 0.01 versus normal control group, ^◆ P < 0.05, ^◆◆ P < 0.01 versus IL-1β and TNF-α combination-treated group.