Figure 5

CGN PE extract induced cellular death is dependent on p53 expression in human breast cancer cells. ZR751 cells were transfected with TP53 siRNA for 24 h. After transfection, cells were exposed to different concentrations of PE extract for 24 h prior to analysis of (A) p53 expression by western blot or (B) cell viability by MTT assay. Each bar represents the mean ± SD (n = 3). Data denoted *(P < 0.05), **(P < 0.01), ***(P < 0.001) and ****(P < 0.0001) are significantly different compared to PE extract treatment alone (without TP53 siRNA treatment) between the same treatment concentration analysed by t-test.