Figure 7

Effect of EA on p-CREB DNA binding activity in L4-6 ipsilateral spinal cord dorsal horn. (A). EMSA experiment showing binding to CREB probe in nuclear extracts prepared from control and model, EA, and sham EA groups at 6 and 49 h after CFA injection. The binding of CREB in the control group was considered for comparison (lane 2). EA regulated CREB protein binding activity at 49 after CFA-treated (lane 7). (B). EMSA experiment showing the binding of proteins present in the nuclear extracts prepared from the model group at 49 h after CFA injection. Specificity of the DNA-protein interaction was assessed by competition EMSA using a 100× molar excess of unlabeled doubled-stranded oligonucleotide CREB probe (lane 3), unlabeled mutant doubled-stranded oligonucleotide CREB probe (lane 4) and biotin-labeled mutant doubled-stranded oligonucleotide CREB probe (lane 5).