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Figure 6 | BMC Complementary and Alternative Medicine

Figure 6

From: Enhancement of gefitinib-induced growth inhibition by Marsdenia tenacissima extract in non-small cell lung cancer cells expressing wild or mutant EGFR

Figure 6

MTE ( M. tenacissima extract) combined with gefitinib reduces EGFR and Met crosstalk in non-small cell lung cancer cells. H292 and HCC827 cells were treated with 1 μM gefitinib, 8 mg/ml MTE, and their combination (MTE → MTE + Gef) in 1% FBS culture medium for 6 h. Cells were stimulates with 10 ng/ml EGF for 15 min before harvest. Cells were lysed and cellular extracts (20 μg protein) were separated on SDS-PAGE gel and transferred to PVDF membranes. Membranes were sequentially probed with Met, phospho-Met, EGFR and phospho-EGFR. β-tubulin was served as a loading control. Blots are representatives of three independent experiments.

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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