Figure 2

AAD reduced total leukocytes and eosinophils in the BALF of allergic mice. The bronchoalveolar lavage fluid (BALF) of mice were harvested at 48 h after the final OVA (or NS) challenge. Subsequently, total leukocytes cell counts (Left) were carried out using a Neubauer chamber. Smears of BALF cells were prepared and eosinophil counts (Right) were performed in light microscopy according to standard morphologic criteria by the smears following Wrights-Giemsa staining, at least 400 cells were counted per slide. The values represent the mean ± SE for 8–10 mice in each group of three independent experiments. H + OVA: OVA challenged and treated with high dose AAD, L + OVA: OVA challenged and treated with low dose AAD. ^#p < 0.05 compared with NS. *P < 0.05 compared with OVA group.