Figure 6

PEE-evoked ER stress in human T24 cell. Human T24 cells were treated with 50 or 100 μg/ml of PEE for different time durations. Various effector proteins participating in ER stress were detected by western blotting with the antibodies against procaspase-12 (A), CHOP (B), BiP (C) and VCP (D) respectively as described in section 2. The blot in each diagram was the representative result of three independent experiments and the densitometer-intensity data (mean ± S.D.) were shown under each blot.