Figure 4

Antioxidant activities of M. grandiflora L. flower extract. (A) ABTS⁺ radical scavenging capacity assay. The flower extract (10, 15, 20%; v/v), vitamin C (50 μM), vitamin E (50 μM) or BHA (0.1 mg/ml) were incubated with ABTS⁺ solution, respectively. (B) Determination of total phenolic content. Different concentrations of the M. grandiflora flower extract (10, 15, 20%; v/v) and gallic acid (2 μg/ml) were used in the assay. (C) Reducing capacity assay. Different concentrations of the flower extract (10, 15, 20%; v/v) or BHA (0.05 mg/ml) were used in the test. (D) Determination of ROS content in B16F10 cells. Cells were treated with various concentrations of the flower extract (10, 15, 20%; v/v) or Trolox (2.0 mM) for 24 h and then the ROS content was measured by the DCF-DA assay. Results are represented as percentages of control, and the data are mean ± S.D. for three separate experiments. Values are significantly different by comparison with control. *** p < 0.001.