Figure 4

The effect of DG on H/R-induced apoptosis in H9c2 cells. After H9c2 cells were challenged by H/R in the presence or absence of DG (125 and 250 μg/ml) or trolox (20 μM), apoptotic cells were analyzed by Annexin V/PI staining. The data were expressed as mean ± S.E.M. Three independent experiments were performed. ^#p < 0.05 and ^###p < 0.001 indicated the groups with significant difference when compared to the normoxic group. *p < 0.05 and **p < 0.01 indicated the groups with significant difference when compared to the control group.