Figure 5From: The roots of Atractylodes japonica Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells Effect of ARA extract on PI3K/IRS-1 signaling pathway in 3T3-L1 cells. The cells were induced to differentiate into adipocytes with MDI medium with or without ARA (100, 200 and 500 mg/mL) for 8 days. The expression of PI3K and the phosphorylation of IRS-1 were analyzed by western blot. Relative density was calculated as the ratio of PI3K and IRS-1 expression to actin expression. Each value represents the meanāĀ±āS.D. (nā=ā3). *pā<ā0.05 vs. control. C: control, 100: ARA 100 Ī¼g/mL, 250: ARA 250 Ī¼g/mL and 500: ARA 500 Ī¼g/mL.Back to article page