Figure 5
Methanolic extracts of G. tenuistipitata (MEGT) induced reactive oxygen species (ROS) changes and glutathione (GSH) depletion of Ca9-22 oral cancer cells. (A) Flow cytometry-based ROS profiles for MEGT-treated cells. Cells treated with different concentrations (0 to 1 mg/ml) of MEGT and H2O2 (100 μM; positive control) for 24 h. (B) Quantification analysis of ROS intensity. Data are presented as mean ± S.E. (n = 3). (C) Flow cytometry-based GSH profiles for MEGT-treated cells. Cells treated with different concentrations (0 to 1 mg/ml) of MEGT and H2O2 (100 μM; positive control) for 24 h. (D) Quantification analysis of intracellular GSH intensity. Data are presented as mean ± S.E. (n = 3). Differences between treatments of different concentrations containing the same letter are not significant.