Figure 3
ROS and NO production by macrophages after treatment. Macrophages were subjected to analyses of reactive oxygen species (ROS) and nitrogen species production. Macrophages were incubated with the specific reaction solution for each assay for different periods. Resulting change in absorbance was determined in a microplate reader. Proper standard concentration curves and positive controls were also done. ANOVA and Tukey test were use to determine statistical differences. We have found that the levels of superoxide anion (O2 -) and hydrogen peroxide (H2O2) release after all treatments have decreased. NO was found to be increased after M1 and M8 treatments. *p < 0.05