Effects of curcuminoids identified in rhizomes of Curcuma longa on BACE-1 inhibitory and behavioral activity and lifespan of Alzheimer’s disease Drosophila models

Background Alzheimer’s disease (AD) is the most common type of presenile and senile dementia. The human β-amyloid precursor cleavage enzyme (BACE-1) is a key enzyme responsible for amyloid plaque production, which implicates the progress and symptoms of AD. Here we assessed the anti-BACE-1 and behavioral activities of curcuminoids from rhizomes of Curcuma longa (Zingiberaceae), diarylalkyls curcumin (CCN), demethoxycurcumin (DMCCN), and bisdemethoxycurcumin (BDMCCN) against AD Drosophila melanogaster models. Methods Neuro-protective ability of the curcuminoids was assessed using Drosophila melanogaster model system overexpressing BACE-1 and its substrate APP in compound eyes and entire neurons. Feeding and climbing activity, lifespan, and morphostructural changes in fly eyes also were evaluated. Results BDMCCN has the strongest inhibitory activity toward BACE-1 with 17 μM IC50, which was 20 and 13 times lower than those of CCN and DMCCN respectively. Overexpression of APP/BACE-1 resulted in the progressive and measurable defects in morphology of eyes and locomotion. Remarkably, supplementing diet with either 1 mM BDMCCN or 1 mM CCN rescued APP/BACE1-expressing flies and kept them from developing both morphological and behavioral defects. Our results suggest that structural characteristics, such as degrees of saturation, types of carbon skeleton and functional group, and hydrophobicity appear to play a role in determining inhibitory potency of curcuminoids on BACE-1. Conclusion Further studies will warrant possible applications of curcuminoids as therapeutic BACE-1 blockers.


Background
Alzheimer's disease (AD) is the most common cause of presenile and senile dementia in developed and developing countries [1,2]. The worldwide prevalence of AD was 26.6 million in 2006, and this figure is projected to grow up to 106.8 million by 2050 [3]. AD is a devastating neurodegenerative disorder of the brain characterized by accumulation and deposition of amyloid β (Aβ) peptide, which are generated by sequential proteolytic processing of transmembrane amyloid precursor protein (APP) by two enzymes, β-secretase (β-site APP cleaving enzyme or BACE-1) and γ-secretase, in the amyloidogenic processing pathways [4,5]. Besides acetylcholinesterase (AChE), BACE-1 is also considered as a key therapeutic target for prevention and treatment of AD [6,7]. Transgenic models of AD in Drosophila melanogaster by driving Aβ production in the central nervous system and retina of the fly have been developed to gain insight into mechanism of AD and to illuminate potential therapeutic approaches [8][9][10]. Although many peptides and heterocyclic compounds have been designed and evaluated as BACE-1 inhibitors [11][12][13], none of them have been successfully developed as AD treatment drugs. There is, therefore, a critical need for the development of new improved anti-AD agents.
Plant secondary substances have been suggested as potential alternatives for AD therapy largely because plants constitute a potential source of bioactive chemicals that have been perceived by the general public as relatively safe and often act at multiple and novel target sites [14,15]. These potential new anti-AD products can be applied to humans in the same manner as conventional anti-AD drugs. Much effort has been focused on them as potential sources of commercial anti-AD products for prevention or treatment of AD. BACE-1 or AChE inhibitors from plants have been well reviewed [13,16] respectively. Recently, plants in the family Zingiberaceae have drawn attention because they contain anti-AD principles [17,18]. The rhizomes of turmeric, Curcuma longa L., are not only important as a spice or flavoring, but they have also been prescribed for indigestion, hepatitis, jaundice, diabetes, atherosclerosis and bacterial infection [19][20][21]. Curcumin, an active ingredient of C. longa, has been proposed to alleviate Aβ toxicity in transgenic human Aβ and human tau flies by reducing the pre-fibrillar/oligomeric species of Aβ [18].
In the current study, an assessment was made of the BACE-1 inhibitory activity of the three curcuminoid compounds isolated from C. longa rhizome (curcumin, demethoxycurcumin, bisdemethoxycurcumin), commercially available curcuminoid compound (tetrahydrocurcumin), a natural BACE-1 inhibitor (EGCG) [22], and a cell-permeable isophthalamide compound (BACE-1 Inhibitor IV) [23], using a fluorescence resonance energy transfer (FRET) enzyme assay. The effects of the two curcuminoids (curcumin and bisdemethoxycurcumin) on feeding, climbing, and life span as well as morphological changes in the compound eyes of D. melanogaster which express human APP and BACE-1 genes within the developing nervous system and compound eyes were compared with those of Inhibitor IV. The mode of anti-AD action and quantitative structure-activity relationship (QSAR) of the curcuminoids also are discussed.

Experimental groups
In our experiment, three curcuminoids isolated from Curcuma longa and one commercial curcuminoid were tested in vitro. Among these compounds, only two curcuminoids, curcumin and bisdemethoxycurcumin, were tested to flies, because the BACE-1 inhibitory activity of demethoxycurcumin in vitro lied between curcumin and bisdemethoxycurcumin. With this issue, demethoxycurcumin was removed from the in vivo study. The experimental groups tested for in vivo study were illustrated in Figure 1. GMR-Gal4 drives target human APP and BACE-1 genes expression in flies' compound eyes, and morphostructural changes of these compound eyes with the supplementation of compounds with different concentration were observed, GMR-Gal4/+ was used as the control group ( Figure 1A), elav-Gal4 drives target genes coexpression in flies' nervous system, behaviors including climbing, life span and feeding assay with the supplementation of compounds with different concentration were tested, elav-Gla4/+ and elav < BACE-1 were used as control groups ( Figure 1B).

Instrumental analysis
1 H and 13 C NMR spectra were recorded in CD 3 CN on a Bruker AVANCE 600 spectrometer (Karlsruhe, Germany) using tetramethylsilane as an internal standard, and chemical shifts were given in δ (ppm). UV spectra were obtained in MeCN on a Kontron UVICON 933/934 spectrophotometer (Milan, Italy) and mass spectra on a Jeol JMS-DX 303 spectrometer (Tokyo, Japan). Merck silica gel (0.063-0.2 mm) (Darmstadt, Germany) was used for column chromatography. Merck pre-coated silica gel plates (Kieselgel 60 F 254 ) were used for analytical thin-layer chromatography (TLC). Merck preparative thin-layer chromatography plates (2 mm thickness) and Biotage Isolera one medium-pressure liquid chromatography (MPLC) (Uppsala, Sweden) were used for isolation of active principles.

Materials
The four commercially-available pure organic curcuminoids examined in this study are listed in Table 1, along with their sources. For the QSAR analysis, values of molecular weight (MW), hydrophobic parameter (log P) and steric effects for the test curcuminoids were obtained from ChemDraw Ultra 10.0 (Cambridge Soft Corporation, Cambridge, MA) and recorded in Table 1. Molecular refraction (MR) was used as the parameter for describing steric effects. Structures of these curcuminoids are given in Figure 1. EGCG was purchased from Sigma-Aldrich (St. Louis, MO), Inhibitor IV, and Acid red were purchased from Merck (Darmstadt, Germany), and Amresco (Cochran Road Solon, OH), respectively. Recombinant human BACE-1 and fluorogenic peptide substrate Mca-SEVNLDAEFRK (Dnp) RR-NH 2 were purchased from R&D system (Minneapolis, MN). All of the other chemicals and reagents used in this study were of analytical grade quality and available commercially.

Plants
The rhizomes of C. longa were purchased from Boeun medicinal herb shop, Kyoungdong market (Seoul, Republic of Korea (ROK)). A voucher specimen (CL-R1) was deposited in the Research Institute for Agriculture and Life Science, College of Agriculture and Life Sciences, Seoul National University.

Fly stocks
Flies were cultured in a standard cornmeal agar medium at 25°C and 70% relative humidity (RH) under a 12:12 h light:dark cycle. Following fly stocks were obtained from Bloomington Stock Center at Indiana University: w1118 (stock number, 3605), UAS-BACE-1, UAS-APP (33797), UAS-BACE-1 (29877), elav-GAL4 (8760), and GMR-GAL4 (1104). The GAL4/UAS system was employed for the overexpression of desired genes in a specific tissue of the fly. The transgenic fly stock UAS-BACE-1 (29877) in our study was also used in previous study [10]. The characterizations of trans-human gene APP and BACE-1 flies as a reliable AD model were presented in results section.

Extraction and isolation
The dried rhizomes of C. longa (1.2 kg) was pulverized and extracted with methanol (3 × 5 L) at room temperature for 2 days and filtered. The combined filtrate was concentrated under vacuum at 40°C to yield~105.4 g of a dark yellowish red tar. The extract (100 g) was sequentially partitioned into hexane-(31 g), chloroform-(6.55 g), ethyl acetate-(49 g), butanol-(3.95 g), and water-soluble (9.5 g) portions for subsequent bioassay. The organic solvent-soluble portions were concentrated to dryness by rotary evaporation at 40°C and the water-soluble portion was freezedried. For isolation of active principles, 2 mg/ml of each Hydrophobic parameter expressed as the log of the octanol/water partition coefficient. c Parameter for steric effects as described using molecular refraction.  C. longa rhizome-derived material was tested in a FRET enzyme assay as described previously [24].
The chloroform-soluble fraction (5 g) was most active and MPLC was performed using a Biotage Isolera apparatus equipped with a UV detector at 254 nm and a column cartridge SNAP (100 g silica gel) with column volume 132 ml. Separation was achieved with a gradient of chloroform and methanol [(100:0 (500 ml), 96:4 (1800 ml), 90:10 (400 ml), 80:20 (400 ml) and 0:100 (500 ml) by volume] at a flow rate 50 ml/min to provide 17 fractions (each about 180 ml). Column fractions were monitored by TLC on silica gel plates developed with chloroform and methanol (95:5 by volume) mobile phase. Fractions with similar R f values on the TLC plates were pooled. Spots were detected by spraying with 2% H 2 SO 4 and then heating on a hot plate. Fractions 8 to 11 (1.5 g) were purified by preparative TLC with chloroform and methanol (95:5 by volume) to yield two active principles 1 (41.7 mg, R f = 0.47) and 2 (27.2 mg, R f = 0.42).

FRET enzyme assay
The previous method [24] was used with a slight modification to assess the BACE-1 inhibitory activity of all compounds. In brief, the assay mixtures containing 1 μl of 0.5 μg/μl recombinant human BACE-1, 0.75 μl of 2.5 μg/ μl fluorogenic peptide substrate, 47.25 μl of 50 mM sodium acetate (pH 4.5), and the constituents (0.1-2000 μg/ ml) in 2% dimethyl sulfoxide (DMSO) were preincubated at 25°C for 1 h followed by adding 16.6 μl of 2.5 M sodium acetate to stop the reaction. Natural BACE-1 inhibitors (EGCG) and Inhibitor IV served as standard references and were similarly prepared. The fluorescence intensity was measured using a Molecular Devices SpectraMAX Gemini XS plate reader (Sunnyvale, CA) at 355 nm excitation and 405 nm emission at room temperature. The inhibition percentage was calculated with the following equation: % inhibition = 100 -[(Fs -Fb)/(Fn -Fb)] × 100, where Fs was the fluorescence of sample, Fb was the fluorescence of the mixture of substrate and DMSO without BACE-1 enzyme, and Fn was the fluorescence of the mixture of BACE-1 enzyme, substrate, and DMSO.

Histological analysis
Cason's trichome staining was performed as described before [25], In brief, the heads of 5-day-old male flies were fixed in 4% paraformaldehyde buffer solution (pH, 7.4) at 4°C overnight, after which, paraffin-embedded preparations of the fly heads were sectioned at 10 μm thickness by using a HM 340E rotary microtome (Thermo Scientific Microm, Walldorf, Germany). Sections were dried at 40°C overnight and subsequently dewaxed with CitriSolv (Fisher Scientific, Fair Lawn, NJ) and rehydrated with a series of ethanol to phosphate-buffered saline solution. Rehydrated paraffin sections were soaked into Cason's trichrome stain for 15 min, and slides were gently swashed in tap water with subsequent wash in distilled water three times. Excess of water was removed and samples were mounted with mounted with a Vectorshield H-1000 mounting medium (Vector Laboratories, Burlingame, CA).
For Congo red staining, sections were dewaxed and then stained in Congo red solution for 12 min, after which sections were rinsed in tap water and dehydrated in 50, 70% ethanol for 1 min, followed by the incubation in 100% ethanol for 4 min. Slides were dried and mounted with mounting medium. Images were observed and captured using EZ4 HD equipped with an Integrated 3.0 Mega-Pixel CMOS camera (Leica, Heerbrugg, Switzerland).

RT-PCR analysis of human APP and BACE-1 genes in transgenic fly
Semi-quantitative RT-PCR was performed to assess the expression levels of human APP and BACE-1 genes in transgenic fly. Total RNA was extracted from the 30-35 heads of 10-day-old male flies using Trizol (Invitrogen Corparation, Carlsbad, CA). RNA and primers were subjected to RT-PCR by using AccuPower RT-PCR Premix (Cat. No. K-2055) (Bioneer, Alameda, CA). This premix contained optimal concentration of all the components necessary for cDNA synthesis and RTase inactivation, as well as amplification in a single 0.2 ml tube. PCR amplifications were performed with specific primers in a total volume of 20 μl containing 2 μl of forward and reverse primer mixture (10 pmol of each primer), 1 μg RNA and DEPC water. The mixture was used for the amplification after initial denaturation at 95°C and 32 cycles (95°C for 30 s, 60°C for 30 s, and 72°C for 30 s). PCR products were visualized by 2% agarose gel electrophoresis containing ethidium bromide. The primer sequences were as follows: for human APP, 5′-GCCGTGGCATTCTTTTG GGGC-3′(forward) and 5′-GTGGTCAGTCCTCGGTC GGC-3′ (reverse) [26]; for human BACE-1, 5′-GCAGG GCTACTACGTGGAGA-3′ (forward) and 5′-GTATCC ACCAGGATGTTGAGC-3′ (reverse) [27]. RP49, which encodes the Drosophila ribosomal protein 49, was used as an internal standard and reference gene using forward and reverse primer pairs 5′-CTGCTCATGCAGAACC GCGT-3′ and 5′-GGACCGACAGCTGCTTGGCG-3′ [26], respectively. The ethidium bromide stained gel image was digitalized using the Molecular Imager Gel Doc XR System (Bio-Rad, Hercules, CA), and calculated by densitometry [28]. Results are presented as relative mRNA expression of each gene to that of RP49 mRNA.

Light microscopy and scanning electron microscopy of adult eyes
Flies were cultured from egg stage on 94 × 25 mm polystyrene vials containing standard media supplemented with each test compound as stated previously. Whole adult flies (1, 24, and 36 days old) were anesthetized in ice and were put on a microscope slide at room temperature for light microscopy. Morphostructural observations of eye were made with a Leica EZ4HD equipped with an Integrated 3.0 Mega-Pixel CMOS camera with 35 × magnification (Hicksville, New York).
For scanning electron microscopy (SEM), ice-anesthe tized flies (36 days old) were attached to a copper mount using silver paint as a conducting adhesive. They were then put directly into the viewing chamber of a scanning electron microscope without prior coating [29]. The external surface eye morphology was visualized by a Carl Zeiss Supra 55VP field-emission scanning electron microscope (Oberkochen, German) at 15 kV.

Life span assay
Groups of 200 newly eclosed male flies equally distributed in 10 vials were incubated in media supplemented with 1 mM CCN, 1 mM BDMCCN, or 200 nM inhibitor IV in 0.1% DMSO. Controls received 0.1% DMSO only. Survivors were transferred to fresh media vials every 4 days. The median survival time (T 1/2 ) as the time when the survivor function equals 50% was determined because median survivorship reflects a more reliable metric than the mean survival time [10]. All treatments were replicated 10 times.

Climbing assay
For the climbing activity, we followed procedure described previously [9,30]. Flies were collected at eclosion and cultured in groups of 20 flies in media supplemented with 1 mM CCN, 1 mM BDMCCN, or 200 nM inhibitor IV in 0.1% DMSO. Flies over-expression APP/ BACE-1 were treated with different concentrations (200, 500, and 1000 μM) of curcuminoids, based on our preliminary test results and previous studies [17,31]. Control flies received media with 0.1% DMSO. Twenty flies were placed in an empty polystyrene D. melanogaster vial (95 mm tall × 24 mm diameter) conjoined with other vial on top and manually banged twice. After 20 s, we counted flies that climbed and crossed the 9.5 cm line from the bottom and calculated the climbing index as the percentage of those relative to the total number of test flies. All trials were replicated five times.

Feeding assay
The adult feeding assay was performed according to previous study [32] with minor modifications. Flies were collected at eclosion and aged in groups of 15 males and 15 females with culture media for 3 days, and then starved for 20 h in vials containing 3 layers of a Whatman no. 2 filter paper (Maidstone, UK) soaked with distilled water. After starvation, flies were transferred onto vials containing the media (with 0.2% Acid red) supplemented with CCN (200, 500, or 1000 μM), BDMCCN (200, 500, or 1000 μM), or inhibitor IV (200 nM) in 0.1% DMSO. Controls were fed with media containing 0.2% Acid red and 0.1% DMSO. After 2 h of feeding, flies were anesthetized, and their abdomens were isolated and homogenized in 1 ml of distilled water. After centrifugation (5000 rpm, 25°C, 5 min), the optical density (OD) of the supernatant was measured at 505 nm. The OD values were as the index of the amount of food taken by flies [33]. All treatments were replicated three times.

Data analysis
Concentrations of the test compounds causing 50% loss of BACE-1 (IC 50 ) were calculated using GraphPad Prism 5.1 software (San Diego, CA). The IC 50 values for each D. melanogaster line and their treatments were considered to be significantly different from one another when their 95% confidence limits (CL) failed to overlap. All data are presented as mean ± standard error, and the significance between means was determined using oneway or two-way analysis of variance (ANOVA) statistical test (GraphPad Prism 5.1 software; San Diego, CA). Statistical analysis for survival data were carried out using the Bonferroni post tests (GraphPad Prism 5.1 software).

FRET bioassay-guided fractionation and isolation of curcuminoids
Fractions obtained from the solvent hydrolyzable of the methanol extract of C. longa rhizomes were examined for inhibitory activity against human BACE-1 using a FRET-based enzyme assay (Table 2). At a concentration of 2 mg/ml, both the methanol extract and chloroformsoluble fractions suppressed completely activity of BACE-1. At 1 mg/ml, the chloroform-soluble fraction was the most potent inhibitory material, followed by the ethyl acetate-soluble fraction. Low and no inhibition were produced by the butanol-and water-soluble fractions, respectively. Therefore, the chloroform-and ethyl acetatesoluble fractions were subjected to further purification steps to identify inhibitory constituents for BACE-1.

In vitro BACE-1 inhibitory activity of curcuminoids
The BACE-1 inhibitory activity of all compounds was likewise compared using in vitro FRET-enzyme assay ( Table 3). Based on IC 50 values, BDMCCN was 20 and 13 times more potent at inhibiting BACE-1 than CCN and DMCCN. The inhibitory activity of DMCCN was significantly different from that of CCN. THCCN was ineffective. Overall, these curcuminoids were significantly less potent at inhibiting BACE-1 than inhibitor IV. BDMCCN was significantly more active than EGCG.

Characterization of trans-human APP and BACE-1 genes fly as a reliable AD model
The GAL4/UAS system was employed for the overexpression of desired genes in a specific tissue of the fly. In our experiment, human APP and BACE-1 genes induced eye degeneration of transgenic fly under GMR-Gal4 driver strain. Control GMR-Gal4/+ flies showed normal Water-soluble fraction 0 0 0  and well-organized compounds eye ( Figure 3A), wellorganized structure and normal retina were stained by Cason's trichome staining ( Figure 3B) and Congo red staining ( Figure 3C and D). However, human APP and BACE-1 co-expression flies presented rough and irregular compound eye ( Figure 3E), damages of retina were showed clearly by Cason's trichome staining ( Figure 3F), and amyloid depositions were observed by Congo red staining marked by with arrows ( Figure 3G and H). APP and BACE-1 were expressed in the nervous system under elav-GAL4 driver strain. We reconfirmed the expression of APP and BACE-1 genes in the transgenic flies with semi-quantitative RT-PCR analysis (Figure 4). Target genes electrophoresis results were observed ( Figure 4A), Gene RP49 was used as the reference gene to normalize mRNA amount, human APP and BACE-1 genes showed high expression amount in transgenic fly, however, no expression in control fly ( Figure 4B). In conclusion, trans-human APP and BACE-1 fly can be used as a reliable AD model.

Effects of curcuminoids on the eye morphology
Morphological defects in flies expressing APP/BACE-1 in compound eye (GMR > Gal4, UAS-APP, UAS-BACE-1 or GMR > APP/BACE-1) were first examined. Control carrying GMR-GAL4 alone showed wild-type eye morphology ( Figure 5A). In contrast, GMR > APP/BACE-1 developed ommatidia atrophia at the edge of compound eye (marked with star in Figure 5B). Next, we cultured GMR > APP/ BACE-1 flies in media supplemented with 1 mM CCN, 1 mM BDMCCN or 200 nM Inhibitor in 0.1% DMSO during entire developmental stages. Even on 1 day posteclosion, the edge atrophia was already ameliorated in the flies cultured in CCN-( Figure 5C1) or BDMCCN-media ( Figure 5D1), compared with the vehicle (0.1% DMSO) control ( Figure 5B1). The manifestation of ommatidia atrophia was also considerably reduced in Inhibitor IV-fed flies ( Figure 5E1). However, any of tested curcuminoids and Inhibitor IV failed to suppress completely the eye degeneration phenotype observed in GMR > APP/BACE-1.
Nevertheless, protective effects of CCN, BDMCCN and Inhibitor IV remained evident in 24 day-and 36-day old flies ( Figure 5). To compare the protective activities of curcuminoids in high resolution, we examined compound eyes of GMR > APP/BACE-1 treated with each compound using scanning electron microscope (SEM). Control flies (GMR-GAL4/+) showed smooth appearance of the eye without any defects of ommatidia size and bristles ( Figure 5A4). In contrast, GMR > APP/BACE-1 flies showed varying degrees of eye disorganization. The eye of flies treated with the vehicle showed the strongest phenotypes characterized by absence of ommatidial bristles and fusion of ommatidia (marked with arrows) ( Figure 5B4). The rough eye phenotype was suppressed partially in 1 mM CCN-fed flies, but most ommatidial bristles were still absent and the size of some ommatidia reduced (arrows in Figure 5C4). Remarkably, protective potency of 1 mM BDMCCN Figure 3 Human APP and BACE-1 genes induced eye degeneration of transgenic fly under GMR-Gal4 driver strain. Control GMR-Gal4/+ flies showed normal and well-organized compounds eye (A), this well-organized structure were stained by Cason's trichome staining (B) and Congo red staining (C and D). Transgenic flies GMR < APP/BACE-1 presented rough and irregular compound eye (E), damages of retina were detected by Cason's trichome staining (F) and amyloid depositions were observed in flies' compound eyes by Congo red staining marked by arrows (G and H). Scale bar, 50 μm.
( Figure 5D4) was comparable to that of 200 nM inhibitor IV ( Figure 5E4).

Effects of curcuminoids on climbing behaviors
Most of neurodegenerative diseases including Alzheimer's disease are characterized by age-dependent deterioration in locomotory coordination. In Drosophila model, the locomotory coordination can be quantified by the negative geotaxis assay, which takes advantage of fly's innate tendency to climb against gravity after gentle tapping. In the assay, control flies cultured on standard media (elav-Gal4/+) showed a clear age-dependent reduction in the climbing indices, for example measured in females as 98%, 93%, 89% and 66% in 1, 10, 20 and 30 days after eclosion, respectively ( Figure 6A). Further, expression of BACE-1 with or without its substrate APP in the nervous system resulted in even stronger age-dependent locomotory deterioration in both genders ( Figure 6). The climbing defect was slightly more pronounced in males than in females, there was virtually no climbing activity scored in elav > APP/BACE-1 males from 20 days after eclosion ( Figure 6B).
Then, we asked whether two curcuminoids and Inhibitor IV BACE-1 blockers could rescue climbing defects in males expressing BACE-1 alone (elav > BACE-1) of three different age groups (10, 20 and 30 days post-eclosion). In 10 days after eclosion, all tested compounds except 1 mM CCN did not significantly improve climbing ability, probably because overall behavioral defect was not pronounced in this age group. In contrast, tested 20 and 30 days after eclosion, elav > BACE-1 males cultured in media containing 1 mM CCN, 1 mM BDMCCN, or 200 nM Inhibitor IV showed significantly improved climbing indices, compared with those cultured in vehicle control (0.1% DMSO) media ( Figure 7B1-B3).
Subsequently, we carried out analogous experiments with elav > APP/BACE-1 males, which show much severe age-dependent progression of locomotory defects. In 10 days post-eclosion, males cultured with CCN (1 mM only), BDMCCN (200 μM, 500 μM, and 1 mM), or inhibitor IV (200 nM) showed less severe impairments in the climbing ability compared with flies cultured in vehicle control ( Figure 7C1). In 20 days post-eclosion, virtually no elav > APP/BACE-1 males can climb against gravity, due to poor movement coordination. However, dietary supplement of 1 mM CCN, 1 mM BDMCCN, or 200 nM Inhibitor IV delayed age-dependent progression of locomotory defects, and improved climbing ability compared with vehicle control ( Figure 7C2). The climbing ability was also partially rescued with 1 mM CCN-and 200 nM Inhibitor IV in 30 days post-eclosion males ( Figure 7C3).
Because feeding behavior is one of the essential factors determining longevity of flies and other animals [35], we examined the effects of compounds on amount of food intake in adults expressing APP/BACE-1 in the nervous . Gene RP49 was used as the reference gene to normalize mRNA amount. For RP49 mRNA expression amount, there was no significant difference between control flies and transgenic flies, however, mRNA of target genes human APP and BACE-1 were not expressed in control flies. ns: no significant difference. Each bar represents standard error.
system (elav > APP/BACE-1). Irrespective of compounds and concentrations examined, CCN, BDMCCN, and Inhibitor IV did not affect amount of feeding in any of tested genotypes (Figure 9). This finding indicates that protective effects of BDMCCN and Inhibitor IV on longevity are not attributed to their possible anorectic effects.
QSAR of BACE-1 inhibitors have been well reviewed previously [11,41]. BACE-1 inhibitory activity of 10 catechins and reported that the inhibitory activity seemed to be related to the pyrogallol moiety on C-2 and/or C-3 catechin skeleton, whereas the stereochemistry of C-2 and C-3 did not have the inhibitory activity [42]. In the current study, absence of methoxy groups in the phenyl rings of CCN increased the BACE-1 inhibitory activity. In particular, absence of two methoxy groups (BDMCCN) significantly increased BACE-1 inhibitory activity. However, THCCN was less potent at inhibiting BACE-1 inhibitory activity than CCN, indicating that the double bonds appear to be essential for the enzyme inhibitory activity. This current finding indicates that structural characteristics, such as degrees of saturation, carbon skeleton, types of functional group, and hydrophobicity rather than MW appear to play a role in determining the BACE-1 inhibitory activity. Previous study [42] also reported that BDMCCN exhibited the most potent inhibitory action on BACE-1 mRNA level, followed by DMCCN and CCN.
Although multiple pathogenetic factors such as Aβ and tau aggregation, excessive metal ions, oxidative stress, acetylcholine level, and increased BACE-1 activity have been suggested for AD, lifestyles and genetic factors also are associated with AD development [43]. The potency of feeding curcumin as a drug candidate to alleviate Aβ toxicity in transgenic Drosophila was also studied [18]. They reported that the longevity and the locomotor activity of five different AD model genotypes showed up to 75% improved lifespan and activity for curcumin-fed flies and any decrease in the amount of Aβ deposition following (See figure on previous page.) Figure 8 Effect of curcumin, bisdemethoxycurcumin, and β-secretase Inhibitor IV supplementation on longevity of elav-Gal4/+, elav < BACE-1, and elav < APP/BACE-1 flies. There was no significant difference in the longevity among three different genotype flies (A). Compound supplementation did not affect longevity of flies elav-Gal4/+ (B), elav < BACE-1 (C), and elav < APP/BACE-1 (D). Median life time of three genotype flies cultured on standard medium (n = 200 flies per group. ***: p < 0.001, **: p < 0.01, *: p < 0.05) (E). Median life time of three genotype flies fed on curcuminoids and BACE-1 Inhibitor IV supplementation media (n = 200 flies per group. **: p < 0.01, *: p < 0.05) (F). Each bar represents standard error. Figure 9 Effect of curcumin, bisdemethoxycurcumin, and β-secretase Inhibitor IV supplementation on adult flies feeding behavior of elav-Gal4/+, elav < BACE-1, and elav < APP/BACE-1 female (A) and male (B) flies. n = 45 flies. Each bar represents standard error. curcumin treatment was not observed. In the current study, we showed prolonged exposure to either CCN or BDMCCN could rescue morphological defects observed in flies expressing APP and BACE-1 in compound eyes (GMR > APP/BACE-1). In addition, dietary supplement of CCN or BDMCCN also improved movement coordination significantly in elav > BACE-1 and elav > APP/ BACE-1 flies, but not in control. Lastly, both BDMCCN and Inhibitor IV increased median life time of elav > BACE-1 and elav > APP/BACE-1 flies. This finding along with BACE-1 inhibitory action indicates that materials derived from C. longa rhizome root may hold promise for the development of novel and effective anti-AD products.