Table 1 The specification of the primers for amplification of the targeted genes
Gene name | Primer sequence (5′ to 3′) | Accession number | Position | Size (bp) | Tm (°C) | Optimized annealing temperature (Ta) (°C) |
|---|---|---|---|---|---|---|
IL-6-F | GTTCGGATAATGTAGCCT | NM_001003301.1 | 633–650 | 135 | 40.6 | 53.9 |
IL-6-R | TCACAGAGAACAACATAACT | 751–768 | 40.5 | |||
CCL-2-F | GTGATCTTCAAGACCGTCCTAA | NM_001003297.1 | 191–212 | 130 | 47.9 | 59.5 |
CCL-2-R | TTCAGAGTGAGTATTCATGGCTT | 299–321 | 46.6 | |||
IFN-β-F | AAACTTCACCTGGGACAA | NM_001135787.1 | 390–407 | 118 | 40.6 | 55.9 |
IFN-β-R | TTTCTGCTTGGACTATTGT | 39.5 |