Fig. 2

EFSF could relieve the anticancer drug-induced neurotoxicity. PC12 cells were differentiated into neural cells in DM for 96 h as described in the Methods, and treated with various concentrations of EFSF (0–400 μg/ml) (a) or with a combination of the indicated anticancer drugs and EFSF (b) for 48 h. c The neural PC12 cells were co-treated with the oxaliplatin (LOHP, 200 nM) and indicated concentrations of EFSF (0–100 μg/ml) or amifostine (Ami, 0.5 mM) as a positive control for 48 h. Cell viability was assessed after 48 h drug treatment. The data from triplicate experiments are presented as the mean ± SD. *** p < 0.001 vs. vehicle, and ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. anticancer drug (b) or LOHP alone (c)