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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: 1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells

Fig. 1

CRACE inhibited differentiation of 3T3-L1 preadipocytes. a. Percent survivability of 3T3-L1 cells as measured by MTT assay after exposure to different doses of CRACE for 24 h. b. CRACE reduced lipid accumulation in differentiating 3T3-L1 cells. Top panel represents the design of the experiment. 3T3-L1 cells were induced with adipogenic cocktail ± CRACE at the displayed doses for 4 days and subsequently kept in maintenance medium for an additional 4 days. Thereafter, ORO staining was performed. Images represent micrographs of ORO stained cells. Scale bar: 100 μm. c. Graph represents absorbance of extracted ORO from different groups of cells represented in panel b. d. Expression of PPARγ1 and PPARγ2 transcripts were reduced in CRACE treated group of induced 3T3-L1 cells. 3T3-L1 cells were induced for differentiation ± CRACE (500 μg/mL equivalent) in the same way as described in panel b. Gel images and bar diagram represent RT-PCR analysis of total RNA isolated after 8 days of induction. e. CRACE reduced PPARγ1, PPARγ2 and PLN1 protein level in differentiating 3T3-L1 cells are represented in panel a. Gel pictures represent western blot analysis of proteins isolated after 8 days of induction. f. CRACE reduced expression of PPARγ target genes. Cell differentiation, and treatment is the same as in panel d. Gel images and bar diagram represent RT-PCR analysis of PPARγ regulated adipocyte factors: PLN1, FABP4, ATGL, LPL, GLUT4, and Adiponectin at 8 days after induction. All bars in the figure represent mean ± SEM, n = 3. p values are from unpaired t-test. * indicates p < 0.05

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