Fig. 5

Effects of ChondroT and its constituent herbs on RANKL-mediated NF-κB activation in BMDMs. BMDMs were pretreated with ChondroT or its constituent herbs for 4 h prior to stimulation with 100 ng/mL of RANKL for 30 min. a NF-κB p65 protein was detected with a polyclonal-anti-NF-κB p65 antibody and Alexa Fluor 488-conjugated anti-rabbit IgG antibody, and then mounted with anti-fade reagent with DAPI. Bay 11–7082 was used as a positive control. b Nuclear fractions were isolated from BMDMs. Protein levels of NF-κB p65 in the nuclear fractions was detected by Western blotting