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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Inhibitory effects of ChondroT and its constituent herbs on RANKL-induced osteoclastogenesis

Fig. 4

Effects of ChondroT and its constituent herbs on RANKL-mediated osteoclast-specific protein expression in BMDMs. BMDMs were pretreated with or without ChondroT or its constituent herbs for 4 h, and then stimulated with 100 ng/mL of RANKL for 48 h. a Expression levels of c-Fos, NFATc1, Cathepsin K, and MMP9 were determined by Western blotting. GAPDH was used as the loading control. Results are the representative of three independent experiments. b-e Protein levels from three independent experiments were quantified using ImageJ software. *P < 0.05; **P < 0.01; ***P < 0.001 versus RANKL group; +++P < 0.001 versus control group; #P < 0.05; ##P < 0.01; ###P < 0.001 versus ChondroT group

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