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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Heracleum moellendorffii roots inhibit the production of pro-inflammatory mediators through the inhibition of NF-κB and MAPK signaling, and activation of ROS/Nrf2/HO-1 signaling in LPS-stimulated RAW264.7 cells

Fig. 4

Effect of HM-R on HO-1 expression through ROS-dependent Nrf2 activation. a RAW264.7 cells were treated with HM-R (50 μg/ml) for the indicated times or indicated concentrations for 6 h. b RAW264.7 cells were treated with HM-R (50 μg/ml) for the indicated times or indicated concentrations for 3 h. After treatment, nuclear fraction was prepared. c RAW264.7 cells were pretreated with NAC (10 mM) for 2 h and then co-treated with HM-R for 6 h for HO-1 analysis or for 3 h for nuclear Nrf2 anaylsis. For Western blot analysis, the cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibodies against HO-1 and Nrf2. Actin or TBP was used as internal control for Western blot analysis. The density of Western blot bands was calculated using the software UN-SCAN-IT gel version 5.1 (Silk Scientific Inc. Orem, UT, USA). *P < 0.05 compared to the cells without the treatment

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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