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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Coix lacryma-jobi var. ma-yuen Stapf sprout extract induces cell cycle arrest and apoptosis in human cervical carcinoma cells

Fig. 4

Effects of Coix lacryma-jobi sprout extract (CLSE) on HeLa cell apoptosis. a–d HeLa cells were stained with FITC-annexin V/propidium iodide (PI) after treatment with various concentrations of CLSE (0–1000 μg/mL) for 72 h, and cell populations and the extent of apoptosis were measured by flow-cytometric analysis. a Representative cell apoptosis dot plots for FITC-annexin V/PI staining in CLSE-treated cells. b-d Quantification of viable cells (FITC-annexin V−/PI−), early-apoptotic cells (FITC-annexin V+/PI−), and late-apoptotic cells (FITC-annexin V+/PI+) using four independent cell populations. e HeLa cells were stained with DAPI (1 μg/mL) after treatment with CLSE (1–1000 μg/mL) for 48 h and observed under a confocal microscope. Arrow, apoptotic cells (magnification: × 400). f HeLa cells were treated with CLSE (1–1000 μg/mL) for 72 h, and apoptosis-related proteins (p53, PARP, Bax, Bcl-2, cleaved caspase-3, and cleaved caspase-8) were examined using western blot analysis. **p < 0.01 and ***p < 0.001 versus control group. All data values are expressed as the mean ± standard deviation (n = 4)

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