Fig. 3

Effect of PGE on cytokine levels in CP-treated splenocytes. Cells were seeded into 96-well plates, followed by treatment with PGE (0, 10, 30, 50, 100, 300, and 500 μg/mL) and/or CP (1600 μg/mL) and incubated for 24 h under 5% CO₂. a–d Level of cytokines (TNF-α, IFN-γ, IL-2, and IL-12) secreted into the culture medium was analyzed using ELISA kits. Bars labeled with different superscripts have significantly different values (P < 0.05). Data are presented as the means ± standard errors (n = 3)