Fig. 7

Effect of VOS on MAPK/ATF2 signaling activation. a RAW264.7 cells were pretreated with VOS for 6 h and then co-treated with LPS (1 μg/ml) for 20 min. b RAW264.7 cells were pretreated with VOS for 6 h and then co-treated with LPS (1 μg/ml) for 30 min. After the treatment, the nucleus fraction was prepared. For Western blot analysis, the cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibodies against p-ERK1/2, p-p38, p-JNK p-ATF2 and ATF2. Total-ERK1/2, total-p38 and total-JNK and actin were used as internal control for Western blot analysis. The density of Western blot bands was calculated using the software UN-SCAN-IT gel version 5.1 (Silk Scientific Inc. Orem, UT, USA). *P < 0.05 compared to the cells without the treatment, and ^#P < 0.05 compared to the cells treated with LPS alone