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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Anti-inflammatory effect of Vaccinium oldhamii stems through inhibition of NF-κB and MAPK/ATF2 signaling activation in LPS-stimulated RAW264.7 cells

Fig. 5

Effect of VOS on osteoclast differentiation. a Effect of VOS on osteoclastogenesis and resorptive activity. The cells were stained with the TRAP assay kit, and media were collected for the TRAP activity. TRAP-positive multinuclear cells were captured using an inverted microscope (100×, Scale bars: 200 mm). The media were measured for TRAP activity by an ELISA reader. b Effect of VOS on the activation of NFATc1 and c-Fos by RANKL. NFATc1 and c-Fos protein expressions were determined by western blot. Expressions of NFATc1 and c-Fos were normalized to actin. c Effects of VOS on the mRNA expression of osteoclastogenesis marker genes by RANKL. The mRNA expressions were detected by RT-PCR and normalized to GAPDH. Columns and error bars represent the mean ± S.D. of three independent experiments. The density of mRNA bands was calculated using the software UN-SCAN-IT gel version 5.1 (Silk Scientific Inc. Orem, UT, USA). *P < 0.05 compared to the cells without the treatment, and #P < 0.05 compared to the cells treated with RANKL alone

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