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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Anti-inflammatory effect of Vaccinium oldhamii stems through inhibition of NF-κB and MAPK/ATF2 signaling activation in LPS-stimulated RAW264.7 cells

Fig. 3

Inhibitory effect of the extracts from stems (VOS), leaves (VOL) and fruits (VOF) of V. oldhami against NO and PGE2 production in LPS-stimulated RAW264.7 cells. a RAW264.7 cells were treated with VOS, VOL or VOF for 24 h. Cell viability was measured using MTT assay. *P < 0.05 compared to the cells without the treatment. b, c RAW264.7 cells were pretreated with VOS, VOL or VOF for 6 h and then co-treated with LPS (1 μg/ml) for 18 h. NO and PGE2 production was measured by Griess assay and Prostaglandin E2 ELISA Kit, respectively. *P < 0.05 compared to the cells without the treatment, and #P < 0.05 compared to the cells treated with LPS alone. d Antioxidant activity and contents of total phenolic compounds of VOS, VOL and VOF were analyzed as described in Materials and methods. e RAW264.7 cells were pretreated with VOS and TA for 6 h and then co-treated with LPS (1 μg/ml) for 18 h. NO production was measured by Griess assay. *P < 0.05 compared to the cells without the treatment, and #P < 0.05 compared to the cells treated with LPS alone

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