Fig. 4

Antibiofilm activity of C. sylvestris extracts from Brazilian biomes against S. mutans and C. albicans. The biomass data obtained via violet crystal method are shown in (a) and (b) for S. mutans and C. albicans, respectively. The microbial viable counts data are shown in (c) and (d) for S. mutans and C. albicans, respectively. Note that the extracts presented more effect against S. mutans biofilms than against C. albicans ones. The data described are median (traces) and interquartis (boxes) of the 12 tested extracts. The error bars represent the maximum and minimum values.Asterisks denote statistically significant difference of a specific extract versus vehicle (V) control, where: ****p ≤ 0.0001; ***p ≤ 0.001; ** ≤ p < 0.01; and *p ≤ 0.05 (Kruskal-Wallis test, followed by Dunn’s multiple comparisons test). Each species growth control is represented as Sm for S. mutans and Ca for C. albicans. The colors of the bars of the graph represent the variety to which the extracts belong, being, in dark gray color: var. sylvestris; light gray: var. intermediate and white: var. lingua