Fig. 3
From: Sinensetin regulates age-related sarcopenia in cultured primary thigh and calf muscle cells
Effect of sinensetin (SIN) on satellite cells of old rats in vitro. After SIN treatment with differentiation medium for 5 days, (a) satellite cells were harvested. MyoD and myogenin protein levels were examined by Western blotting. The level of α-tubulin was used for normalization using Image J program. Data in graphs are presented as mean ± SEM. Statically significance was determined with ANOVA followed by a Dunnett’s test; #p < 0.05 vs. young, *p < 0.05 vs. old with no SIN. b Differentiated satellite cells were immunohistochemically stained for myogenin in green and DAPI in blue. These images were captured with a confocal microscope (X1,000)