Fig. 2
From: Sinensetin regulates age-related sarcopenia in cultured primary thigh and calf muscle cells
Morphology changes of isolated satellite cells. Isolated satellite cells were cultured in proliferation medium for 5 days. Differentiation medium was then provided to both young and old groups. Old group cells were treated with sinensetin (SIN) at indicated concentrations in differentiation medium. a Morphological changes of satellite cells were observed after 5 days of treatment with SIN at indicated concentrations (50 and 100 μM). The observation was done using a light phase-contrast microscope (X200). b Myoblast length was measured using Image J software (U.S. National Institutes of Health, Bethesda, MD, USA). Data are expressed in graphical representation with improved length at indicated concentrations of SIN. Data in graphs are presented as mean ± SEM. Statically significance was determined with ANOVA followed by a Dunnett’s test; ###p < 0.05 vs. young, ***p < 0.05 vs. old with no SIN