Fig. 3
Influence of EE extract and EF and AF fractions on the proliferation of activated lymphocytes. VPD 450-labeled human lymphocytes (5 × 105 cells) were cultured in the presence of medium, dexamethasone (DEXA, 8 μg/mL) or different concentrations of EE (7.5 and 15 μg/mL), EF (15.0 and 25 μg/mL), AF (25.0 and 50.0 μg/mL), and activated with PHA (5 μg/mL) for 5 days. The lymphocyte proliferation was analyzed using flow cytometry. The results are expressed as the mean ± SD of the proliferation index of human lymphocytes (n = 6). The ANOVA statistical method was used, followed by Tukey’s post hoc. * means significant difference (p < 0.05)