Fig. 3

Puerarin reverses RANKL-enhanced OCP autophagy. a Following treatment with or without Puerarin (50 μM) for 6 h in the presence of RANKL, the protein level of Atg7, Beclin1 or Atg5, and the ratio of LC3II/I were detected by Western Blotting. b After treatment as described for (a), LC3-puncta in each group was imaged using the immunofluorescence staining and observed using the fluorescence microscope. Scale bar, 20 μm. c Statistical diagrams exhibited the percentages of LC3-puncta positive cells (more than five dots, 50 cells/field, n = 5). Data are presented as mean ± SEM from three independent experiments. Compared between control group and RANKL group, RANKL group and RANKL+PR group, respectively, *P < 0.05. ns, no significance; PR, Puerarin; Cont, control groups