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Table 2 Method validation for the quantitative determination of four compounds using RP-HPLC

From: Chemical composition, antibacterial activity and related mechanism of valonia and shell from Quercus variabilis Blume (Fagaceae) against Salmonella paratyphi a and Staphylococcus aureus

Peak no.

Compounds

Retention time

Regression equation

Precision experiment

Repeatability

Recovery experiment

Area of peak

RSD (%)

Area of peak

RSD (%)

Average recovery rate

RSD (%)

1

tannin acid

6.794

Y = 19,106x + 31.263 (R2 = 0.9996)

2492.6 ± 10.1

0.41

159.4 ± 33.44

2.09

105.57 ± 1.75

1.36

2

theophyline

15.605

Y = 30,252x-13.334 (R2 = 0.9995)

3753 ± 5.9

0.20

1346.8 ± 53.74

2.22

102.86 ± 1.25

1.18

3

caffeic acid

27.98

Y = 31,908x + 31.065 (R2 = 0.9994)

4172.2 ± 8.2

0.20

625.26 ± 17.25

2.75

98.36 ± 0.36

0.34

4

ellagic acid

35.447

Y = 206,229x + 1865.8 (R2 = 0.9984)

22,707.5 ± 103.3

0.34

20,841.7 ± 232.01

1.11

102.54 ± 2.83

2.75

  1. Values were expressed in mean ± SD (n = 6).