Fig. 3From: Ethanol extract of Chrysanthemum zawadskii Herbich induces autophagy and apoptosis in mouse colon cancer cells through the regulation of reactive oxygen speciesEthanol extracts of Chrysanthemum zawadskii Herbich (ECZ) induced autophagy in mouse colon cancer CT-26 cells. a Detection of acidic vesicular organelles (AVOs). b Representive graph of autophagic celss. Cells were treated with various concentrations (100–300 μg/ml) of ECZ for 24 h and stained with 1 μM acridine orange (AO) at 37 °C in the dark for 20 min and then analyzed by flow cytometry. c Expression of LC3B and p62/SQSTM1. d Relative fold change of LC3B-II. e Relative fold change of p62/SQSTM1. Total cell lysates were analyzed by Western blotting. f Pretreatment with 3-MA reversed the ECZ-induced increase in autophagy. g Effect of 3-MA on LC3B and p62/SQSTM1 expression. h Relative fold change of LC3B-II. (I) Relative fold change of p62/SQSTM1. Cells were pretreated with 1 mM 3-MA prior to 1 h and AO-stained cells were evaluated by flow cytometry. Total lysates were subjected to SDS-PAGE for Western blot analysis. Data are presented as the mean ± standard deviation for at least three independent experiments. **p < 0.01, ***p < 0.001 vs. the control group; #p < 0.05, ##p < 0.01 vs. the ECZ treated groupBack to article page