Fig. 1

ACT doesn’t affect primary chondrocyte cell viability. Rat primary chondrocytes were isolated and cultured in vitro. a Cell morphologies of chondrocytes were examined with a microscope. b Toluidine blue staining of primary rat chondrocytes. c Collagen II staining for primary rat chondrocytes were performed to identify the chondrocytes. d The molecular structure of ACT was shown. e Primary rat chondrocytes were treated with ACT (0, 10, 50, 100 μM) for 24 h, CCK-8 assay was employed to detect cell viability. a-c were representative data from three independent experiments. e was representative result from three independent experiments with triplicates