Fig. 2

Combinatory effects of SN extracts and gemcitabine on PDAC. The efficacy of drug combinations was assessed by treating cells in an 8 × 8 matrix combination of SN and gemcitabine that was 2-fold serially diluted from the highest concentration of 50 mg/L. Cell proliferation was evaluated in 96-well plates using MTT assay at 72 h after treatment. Combenefit software was used to generate and assess the dose-response surface curves and drug interaction effects following the HSA model and Bliss independence model as implemented in the software. The level of synergy (blue) or antagonism (red) at each combination was indicated by the color scale shown. The net Bliss interaction volumes of the matrix drug combination were calculated based on MacSynergy II program and graded accordingly. All the experiments were conducted in triplicate and performed independently