Fig. 4From: Anti-inflammatory activities of astringent persimmons (Diospyros kaki Thunb.) stalks of various cultivar types based on the stages of maturity in the Gyeongnam provinceInhibitory effects of various stalk extracts on NO production in LPS-induced RAW 264.7 cells. Cells were pretreated with various concentrations (0, 10, 20, 50, 100 μg/ml) of stalk extracts for 1 h and then LPS (1 μg/ml) was added and incubated for 24 h. After 24 h, the nitrite concentrations in medium were determined by NO Detection Kit. The results are presented as the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001Back to article page