Glands | Relative intensity of immune-reactive protein bands (enzyme/actin) |
|---|
Px | SOD |
|---|
Control | 1.87 ± 0.1 | 1.40 ± 0.10 |
STZ | 1 ± 0.1 ** | 1 ± 0.1* |
STZ + NDGA | 2 ± 0.2 # | 1.47 ± 0.06 # |
STZ + AE | 3.8 ± 0.4 ## | 2.8 ± 0.3 ## |
- The relative intensity was determined by densitometry analysis of immunoreactive bands with a software. Control: without treatment, STZ streptozotocin, NDGA nordihydroguaiaretic acid, AE aqueous extract of L. divaricata, Px peroxidase, SOD superoxide dismutase. Results represent Mean ± SEM of three determinations made by duplicate. * P < 0.05, ** P < 0.01significantly differences between submandibulary glands from normal control and submandibulary glands from STZ- treated animals, # P < 0.05, ##P < 0.01 significantly differences between glands from STZ control and STZ + AE or NDGA treated glands in accordance with ANOVA+ Dunnett’s test. AE: 500 μg/ml, NDGA: 1.5 μg/ml
