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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: In vitro and in vivo effects of traditional Chinese medicine formula T33 in human breast cancer cells

Fig. 3

Detection of autophagy in MDA-MB231 and MCF-7 cells. Representative images of immunofluorescence staining of LC3-II specific antibodies in MDA-MB231 and MCF-7 cells that were treated with different concentrations of T33 for 48 h and reacted with DAPI (left panel) and antibodies against LC3-II (middle panel). Right panel indicates the merged images of DAPI and LC3-II. An arrow indicates the expression of LC3-II. Similar results were observed in triplicate experiments. Scale bars = 20 μm

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